|
Bacterial and Virus Strains
|
|
E. coli BL21 (DE3) |
Invitrogen |
EC0114 |
|
Chemicals, Peptides, and Recombinant Proteins
|
| 7H11 |
Millipore-Sigma |
M0428 |
| 7H9 |
HIMEDIA |
M198 |
| AAC(2’)-Ic |
Generated in our lab |
Ref 17
|
| AAC(3)-IV |
Generated in our lab |
Ref 28
|
| AAC(6’)-Ie |
Generated in our lab |
Ref 28
|
| AcCoA trilithium salt |
CoALA Biosciences |
AC02 |
| Ammonium sulfate |
Fisher Scientific |
BP212-212 |
| Ampicillin, sodium salt |
Millipore-Sigma |
A9518 |
| Casaminoacids |
VWR |
J851 |
| CoA, trilithium salt |
Millipore-Sigma |
C3019 |
| DMSO |
TCI |
D0798 |
|
d-pantothenic acid hemicalcium salt |
Millipore-Sigma |
P2250 |
| DTNB |
Millipore-Sigma |
D8130 |
| EDTA dibasic |
Millipore-Sigma |
E5134 |
| Eis |
Generated in our lab |
Ref 17
|
| Eis C204A |
Generated in our lab |
Ref 17
|
| Glycerol |
Millipore-Sigma |
G7893 |
| HCl |
Millipore-Sigma |
258148 |
| HEPES free acid |
Millipore-Sigma |
H3375 |
| Imidazole |
Millipore-Sigma |
I202 |
| IPTG |
GoldBio |
I2481C |
| Isoniazid |
Millipore-Sigma |
I3377 |
| Kanamycin sulfate |
VWR |
O408 |
| LB broth |
VWR |
J106 |
| McFarland standard |
Pro-Lab Diagnostics |
SD2300 |
| Neomycin trisulfate |
Millipore-Sigma |
N1876 |
| OADC |
Prepared in our lab |
Ref 42
|
| PEG 8000 |
Millipore-Sigma |
89510 |
| Resazurin, sodium salt |
Millipore-Sigma |
199303 |
| Screening plate/compounds |
|
|
| Sodium chloride |
Millipore-Sigma |
S9888 |
| Sodium hydroxide |
Millipore-Sigma |
221465 |
| Sodium phosphate dibasic |
Millipore-Sigma |
S9763 |
| Sodium phosphate monobasic |
Millipore-Sigma |
331988 |
| Triethanolamine hydrochloride |
Millipore-Sigma |
T1502 |
| Tris |
Millipore-Sigma |
T1503 |
| Tyloxapol |
Millipore-Sigma |
T0307 |
|
Deposited Data
|
| Eis crystal structure |
Protein Data Bank |
3R1K |
|
Experimental Models: Organisms/Strains
|
|
Mtb mc26230 |
Provided by William Jacobs (Albert Eistein College of Medicine, USA) |
|
|
Mtb mc26230 K204 |
Generated in our lab |
Ref 19
|
|
Oligonucleotides
|
| Int-pET19b-pps |
Generated in our lab |
Ref 29
|
| pET22b |
Millipore-Sigma |
69744 |
| pET28a |
Millipore-Sigma |
69864 |
|
Software and Algorithms
|
| CCP4 suite |
https://www.ccp4.ac.uk/
|
|
| COOT |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
|
| HKL2000 |
https://hkl-xray.com/hkl-2000
|
|
| JLigand |
Included in CCP4 suite |
|
| MOLREP |
Included in CCP4 suite |
|
| Phaser |
Included in CCP4 suite |
|
| SigmaPlot 15.0 |
SYSTAT |
|
|
Other
|
| Ni-NTA resin |
Qiagen |
30250 |
