Fig 7.

Dasatinib rescues detection of HIV infection with activated T cell co-culture in CellRaft arrays. MOA cells were plated in CellRaft arrays at four cells per raft and then infected with HIV (NL4-3). At 2 hours post infection, media were replaced with fresh media alone or media containing PHA/IL-2-activated T cells at 50 cells per raft with or without 10 nM Das. In one set of arrays, the media with Dasatinib were replaced at 24 hours with media containing control vehicle. At day 4 of co-culture, the arrays were scanned and the quantity of infected rafts (mCherry positive) was quantified for each condition. Representative images from the arrays are shown in A. Quantified results are shown in B Each bar represents the average of three replicates. Error bars represent the standard deviation of the mean. Asterisk indicates statistically significant difference between the conditions (P < 0.05)