Fig 9.

Detection of HIV-infected cells in PWH on ART. Resting CD4 T cells from seven PWH on ART and four serogenative controls were activated for 48 hours with PHA/IL-2, then washed, and co-plated in hexaquad CellRaft arrays at 50 T cells per raft with MOA cells (4 cells per raft) in the presence of 10 nM Dasatinib. Arrays were scanned daily for 7 days of co-culture. (A) Examples of two rafts with evidence of viral transmission are shown. (B) Comparison of MOA IUPM values for seronegative (blue) and HIV-seropositive donors (red). P value calculated by Mann-Whitney U test. (C) Quantification of total mCherry+ rafts for each donor over the timecourse calculated as IUPM and compared with known QVOA IUPM values for the same donors. Pearson correlation r² and statistical significance are shown. Serostatus for HIV indicated by color—blue for seronegative and red for seropositive.