Fig 3.

Assembled particle formation in wild-type and øX174 mutant infected cells. Extracts made from infected cells were analyzed by the rate of zonal sedimentation. Gradients were fractionated from the bottom of the tube. Thus, lower fractions contain particles with higher S values. Mutant names and infection temperatures are given in the figure. (A and B). Mutants that produce virions but at reduced yields compared to wild type. (C) Mutants that do not produce soluble assembled particles. The sedimentation profiles of mutants l(J)Y31E, l(J)W30R, l(J)Y31M, and L(J)Y31L are not depicted but yielded similar results. (D). Mutants that produced particles that sediment at ~70S.