Fig 4.

Composition of assembled particles generated by the l(J)F37E and l(J)Y31V packaging mutants. (A) SDS-PAGE of gradient fractions from Fig. 3D. Only the region of the gel with the viral coat protein is depicted. Band intensity corresponds to the A260 readings reported in Fig. 3D. V indicates purified viral coat protein. (B) DNA isolated from 114S and 70S gradient fractions. Symbols: ss, purified single-stranded DNA genomes; RF, replicative form dsDNA. The bracketed band identifies the small aberrant ssDNA associated with the l(J)Y31V 70S material. (C) PCRs were conducted with wild-type and l(J)Y31V 70S DNA. The six PCRs span the entire genome. Reaction numbers utilized the same primers for each template. Bracketed bands identify PCR fragments unique to the l(J)Y31V 70S DNA template.