TABLE 1.
Allele specificity of the S1F and D209H suppressors in the coat protein
| Parental mutant | Parental phenotype | EOPa with and without suppressors | Other coat suppressorsb | ||
|---|---|---|---|---|---|
| Nonec | S1F | D209H | |||
| W30F | cs | 10−4 | 10−6 | 1.6 | P93F |
| W30Yd | weak cs | 10−1–10−2 | 0.2 | 0.8 | L324F |
| W30H | lethal | 10−4 | 0.1 | 10−5 | |
| W30V | lethal | 10−4 | 10−3 | 10−5 | L324F |
| W30N | lethal | 10−5 | 10−6 | 10−5 | |
| W30E | lethal | <10−3 | NDa | ND | |
| W30R | Lethal | 10−6 | 10−6 | 10−6 | |
| Y31F | WT | 1.0 | NAa | NA | |
| Y31W | cs | 10−3 | 10−4 | 0.6 | Q23H |
| Y31H | ts | 10−4 | 1.1 | 1.1 | |
| Y31V | lethal | 10−4 | 0.5 | 1.0 | L324F |
| Y31N | lethal | 10−3 | 0.5 | 10−6 | |
| Y31E | lethal | 10−4 | 10−5 | 10−5 | |
| Y31R | lethal | 10−5 | 10−6 | 10−5 | |
| Y31Md | lethal | 10−4 | 0.4 | 1.0 | N90D, A230G, L235I |
| F37Y | WT | 1.0 | NA | NA | |
| F37W | WT | 0.5 | NA | NA | |
| F37H | ts | 10−4 | 0.3 | 0.3 | |
| F37V | WT | 1.0 | NA | NA | |
| F37N | lethal | 10−3 | 1.2 | 0.5 | L236F |
| F37E | lethal | 10−4 | 0.8 | 0.8 | D65G |
| F37R | lethal | 10−5 | 10−5 | 10−5 | |
a
EOP (efficiency of plating restrictive titer/permissive titer). ND: not determined. NA: not applicable.
b
Other coat protein mutations that suppressed the defective phenotype.
c
The EOP values were determined under restrictive conditions of the parental mutant.
d
The W30Y mutant was rescued by an intragenic suppressor Q35R, an extragenic suppressor in the external scaffolding protein T111I, and a mutation in the gene E (T74M), which encodes the lysis protein. Y31M mutation was also suppressed by a substitution in the external scaffolding protein V3F.