Fig. 3.

SDS-PAGE analysis of recombinant Escherichia coli expression products. M, PageRuler Prestained Protein Ladder; 1, expression products of E. coli BL21(DE3)/pACYCDuet-1; 2, crude expression product of E. coli BL21(DE3)/pACYCDuet-1-Ectdh; 3, crude expression product of E. coli BL21(DE3)/pACYCDuet-1-Ectdh-Ehnox; 4, crude expression product of E. coli BL21(DE3)/pETDuet-1; 5, crude expression product of E. coli BL21(DE3)/pACYCDuet-1-Ectdh-Ehnox: pETDuet-1-Scaao; 6, crude expression product of E. coli BL21(DE3)/pACYCDuet-1-Ectdh-Ehnox: pETDuet-1-Scaao-Ecsstt