TABLE 2.
Reactivity of MAb 19 with S1-, S1S-, and S2-MAP constructs and respective equilibrium constants
| MAP construct | ELISA resulta | Inhibition by monomeric peptideb | Inhibition by RSV (%)c | Biosensor K (M−1)d |
|---|---|---|---|---|
| S1-MAP | 1.15 | NS | 75.3 | 1.19 × 109 (± 0.23) |
| S1S-MAP | 1.57 | NS | 68.8 | 4.93 × 109 (± 0.31) |
| S2-MAP | <0.1 | NA | NA | NB |
a
ELISA results are presented as absorbance obtained with MAb 19 at 30 ng/ml.
b
Inhibition with the corresponding monomeric peptide was performed with a concentration of S1 or S1S of 0.5 mg/ml in the liquid phase. NS, not significant; NA, not applicable.
c
Inhibition with sucrose gradient-purified RSV was obtained with a concentration of 30 μg/ml (total protein concentration) in the liquid phase; no inhibition was detected when the same concentration of measles virus was used.
d
Results are the means of three different experiments (± standard deviations). NB, no detectable binding.