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MAGI cell assay of wild-type and mt4 mutant viruses
| Virus stock | Preparation temp (°C) | No. of blue cells assayed ata:
|
||
|---|---|---|---|---|
| 34.5°C | 37°C | 39.5°C | ||
| mt4 | 34.5 | 395 | 23,900 | 3,900 |
| Wild type | 34.5 | 583 | 23,200 | 4,010 |
| mt4 | 39.5 | 2 | 13 | 10 |
| Wild type | 39.5 | 513 | 5,910 | 5,350 |
MAGI cells were infected with similar amounts (approximately 5 ng of p24gag) of the indicated virus preparations. Only cells containing dark blue nuclei were counted as positive; each value is the average from two independent experiments.
