FIG. 5.

Effect of different lipid and protein kinase inhibitors on adenovirus internalization. SW480 cells were pretreated in suspension for 10 min at 4°C with 100 nM wortmannin (WTM) or were treated with 100 μM LY294002, 2 μM ML7, or 20 μM PD98095 or in medium alone (control) for 2 h at 37°C before detachment. The cells were then incubated with ¹²⁵I-labeled adenovirus for 30 min at 4°C, washed, and warmed to 37°C for various times prior to assaying for virus internalization as measured by resistance to trypsin treatment.