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. 2024 Mar 19;221(5):e20231359. doi: 10.1084/jem.20231359

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© 2024 Zhao et al.

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Figure S2. — Evaluation of metastatic potential of SC-PDCC lines and pooled PDCCs, related to Fig. 2. (A) In vitro invasion assay of six SC-PDCC lines in P2. The invaded cells were counted in five randomly chosen areas. Moderate metastatic potential (MM) compared with low metastatic potential (LM) group; ****, P < 0.0001; t test, two-tailed; error bars represent SD of the mean. Scale bar, 100 µm; n = 6. (B) In vitro invasion assay of nine SC-PDCC lines in P3. ***, P < 0.001; ****, P < 0.0001; t test, two-tailed. Error bars represent SD of the mean. Scale bar, 100 µm; n = 9. HM, high metastatic potential. (C) Representative H&E staining and KI67 staining images of liver metastases derived from pooled PDCC lines of P5 with diagnosed liver metastasis. Scale bar: up, 500 µm; down, 50 µm.