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. 2023 Nov 3;10(1):e1318. doi: 10.1002/vms3.1318

FIGURE 2.

FIGURE 2

Specificity and repeatability of the established loop‐mediated isothermal amplification (LAMP) and loop‐mediated isothermal amplification−lateral flow dipstick (LAMP–LFD) methods. (a) Real‐time fluorescence curves of LAMP; the black curve is the amplification curve of Clostridium piliforme; (b) repeatability of LAMP; numbers 1−4 represent 2.5, 2.5 × 10−1, 2.5 × 10−2 and 2.5 × 10−3 ng/μL, respectively; (c) detection via LAMP–LFD: Numbers 1−24 are Listeria monocytogenes, Salmonella enterica, Shigella dysenteriae, Salmonella typhimurium, Popoff serovar Choleraesuis, S. enterica, Muribacter muris, Salmonella enterica subsp. enterica, Bordetella bronchiseptica, Edwardsiella tarda, Escherichia coli. Corynebacterium kutscheri, Streptococcus suis, Klebsiella oxytoca, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Aeromonas hydrophila, Salmonella paratyphi A, Pasteurella pneumotropica, Shigella boydii, Shigella flexneri, Klebsiella sp., Salmonella enterica subsp. enterica serovar Pullorum. P: C piliforme, N: negative control.