Table 1.
Antibodies used in this study.
| Target | Antibody/Clone Name | Species, Isotype | Label | Source | Use | Comments |
|---|---|---|---|---|---|---|
| CD11b, human | 44a | mouse IgG2a | (-) | E. Hewlett | Function Blocking | Purified at UVA Hybridoma Core; no azide |
| CD11b, human | ICRF44 | mouse IgG1 | (-) | Biolegend | Function Blocking | LEAF™ Purified |
| CD11b, human | ICRF44 | mouse IgG1 | PE | eBiosciences | Flow cytometry | Reports total surface CD11b |
| CD11b, human and mouse | M170 | rat IgG2b, κ | (-) | E. Hewlett | Function Blocking | Purified at UVA Hybridoma Core; no azide |
| CD11b, human and mouse | M170 | rat IgG2b, κ | PE | Biolegend | Flow cytometry | |
| CD11b, active, human | CBRM1/5 | mouse IgG1 | APC | Biolegend | Flow cytometry | Reports active conformation of CD11b |
| CD18, human | TS1/18 | mouse IgG1, κ | (-) | Thermo Fisher Scientific | Function Blocking | LEAF™ Purified |
| CD11a, human | HI111 | mouse IgG1, κ | (-) | Biolegend | Function Blocking | LEAF™ Purified |
| CD11c, human | Clone 3.9 | mouse IgG1, κ | (-) | Biolegend | Function Blocking | LEAF™ Purified |
| C3, human | Anti-human C3 | goat | FITC | MP Bio/Cappel | IF, Western blot | |
| C3b/iC3b, human | 3E7 | mouse IgG1 | (-) | R. Taylor | Function Blocking, Flow Cytometry | Purified at UVA Hybridoma Core |
| N. gonorrhoeae | rabbit | (-) | Meridian | Flow cytometry, IF | Labeled in-house with DyLight 650, Alexa Fluor 555, or Alexa Fluor 647 | |
| Opa proteins | 4B12 | mouse IgG2a | (-) | Criss lab stock | Western blot | Purified at UVA Hybridoma Core |
| CD63, human | AHN16.1 | mouse | FITC | Ancell | Flow cytometry | |
| CD66b, human | CLB-B13.9 | mouse | FITC | Sanquin Pelicluster | Flow cytometry | |
| CD14, human | MΦP9 | mouse IgG2b | BV711 | Becton Dickinson | Flow cytometry | For neutrophil purity |
| CD49d, human | 9F10 | mouse IgG1, k | PE | Biolegend | Flow cytometry | For neutrophil purity |
| CD16, human | 3G8 | mouse IgG1, k | BV421 | Biolegend | Flow cytometry | For neutrophil purity |
| CEACAMs, human | D14HD11 | mouse IgG1 | (-) | Origene | Function Blocking | |
| Ly6G, mouse | 1A8 | rat IgG2a, κ | FITC | Biolegend | Flow cytometry | |
| Lactoferrin, human | rabbit | (-) | MP Bio/Cappel | IF | ||
| Neutrophil elastase, human | AHN10 | mouse IgG1, κ | (-) | Sigma-Aldrich | IF | Labeled in-house with AF555 (Thermo Fisher) |
| Talin, human | 8D4 | mouse IgG1 | (-) | Sigma-Aldrich | IF | Labeled in-house with AF555 (Thermo Fisher) |
| IgG, rabbit | goat | AF555 | Thermo Fisher Scientific | IF | Highly cross-absorbed | |
| IgG, rabbit | goat | AF488 | Thermo Fisher Scientific | IF | Highly cross-absorbed | |
| IgG, mouse | goat | AF488 | Thermo Fisher Scientific | IF | Highly cross-absorbed | |
| IgG, mouse | goat | AF647 | Thermo Fisher Scientific | IF | Highly cross-absorbed | |
| Isotype control | MOPC-21 | mouse IgG1 | FITC | eBiosciences | Flow cytometry | |
| Isotype control | MOPC-21 | mouse IgG1 | APC | Biolegend | Flow cytometry | |
| Isotype control | P3.6.2.8.1 | mouse IgG1 | PE | eBiosciences | Flow cytometry | |
| Isotype control | 15H6 | mouse IgG1 | (-) | Southern Biotech | Function Blocking | |
| Isotype control | HOPC-1 | mouse IgG2a | (-) | Southern Biotech | Function Blocking | |
| Isotype control | RTK4530 | rat IgG2b, κ | PE | Biolegend | Flow cytometry | |
| Akt, human | E7J2C | mouse | (-) | Cell Signaling | Western blot | |
| Phospho-Akt (Thr308) | C31E5E | rabbit mAb | (-) | Cell Signaling | Western blot | |
| Erk1/2, human | 3A7 | mouse mAb | (-) | Cell Signaling | Western blot | |
| phospho-Erk 1/2 (MAPK Thr202/Tyr204) | 9101 | rabbit mAb | (-) | Cell Signaling | Western blot | |
| p38, human | 9212 | rabbit mAb | (-) | Cell Signaling | Western blot | |
| phospho-p38 (Thr180/Tyr182) | 28B10 | mouse mAb | (-) | Cell Signaling | Western blot | |
| Mouse IgG (H+L) | goat | DyLight 800 | Thermo Fisher Scientific | Western blot | ||
| Rabbit IgG (H+L) | goat | AF680 | Thermo Fisher Scientific | Western blot |