Figure 2.

Human ANGPTL7 levels induced by steroid treatment. (A) HTM cells were treated with 100-nM Dex or ethanol vehicle for 5 days, and ANGPTL7 gene expression was measured by qPCR. Expression of ANGPTL7 in the presence of Dex was normalized to expression in the presence of vehicle. Data are shown as mean ± SEM (n = 2 or 3). (B) Human ANGPTL7 was quantified in aqueous humor samples using ELISA (*P < 0.05, **P < 0.01, ***P < 0.001, Kruskal–Wallis test followed by Dunn's multiple comparison test). Control and glaucoma samples without steroids were not statistically different (Kruskal–Wallis test followed by Dunn's multiple comparison test). Samples below the limit of quantitation (LOQ) are entered as 0.5× the LOQ (0.32 ng/mL). Data are shown as mean ± SEM.