FIG. 5.

Expression of SHDAg does not affect the transactivation activity of LHDAg. One microgram of 3×Sp1CAT DNA was transfected with a fixed amount (4 μg) of a plasmid expressing LHDAg and increasing amounts of a plasmid expressing SHDAg into HepG2 cells. The molar ratio of SHDAg over LHDAg DNA is indicated; pcDNA3 plasmid DNA was added to each transfection to bring the total amount of DNA used in each transfection to a constant level. At 48 h posttransfection, CAT activity was determined and normalized to the level produced in the presence of LHDAg alone, which was set at 1.0.