KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-PPARγ | Cell Signaling Technology | Cat#2435; RRID: AB_2166051 |
| Rabbit anti-C/EBPα | Cell Signaling Technology | Cat#8178; RRID: AB_11178517 |
| Rabbit anti-FABP4 | Cell Signaling Technology | Cat#3544; RRID: AB_2278257 |
| Mouse anti-AKT(pan | Cell Signaling Technology | Cat#2920; RRID: AB_1147620 |
| Rabbit anti-p(S473) AKT | Cell Signaling Technology | Cat#4060; RRID: AB_2315049 |
| Rabbit anti-p(T308)AKT | Cell Signaling Technology | Cat#9275; RRID: AB_329828 |
| Mouse anti-CREB | Cell Signaling Technology | Cat#9104; RRID: AB_490881 |
| Rabbit anti-p(S133)CREB | Cell Signaling Technology | Cat#9198; RRID: AB_2561044 |
| Rabbit anti-IRβ | Cell Signaling Technology | Cat#3025; RRID: AB_2280448 |
| Rabbit anti-p(Y1131)IGF1β/ p(Y1146)IRβ | Cell Signaling Technology | Cat#3021; RRID: AB_331578 |
| Rabbit anti-c-Myc | Cell Signaling Technology | Cat#9402; RRID: AB_2151827 |
| Mouse anti-ACLP/AEBP1 | Santa Cruz Biotechnology | Cat#sc-271374; RRID: AB_10608716 |
| Mouse anti-α-Tubulin | Sigma | Cat#T6199; RRID: AB_477583 |
| Mouse anti-Periostin | Sigma | Cat#SAB4200197; RRID: AB_10669144 |
| Rabbit anti-Igfbp2 | Fisher Scientific | Cat#MAB797; RRID: AB_2264599 |
| Rabbit anti- αSMA | Abcam | Cat#ab5694; RRID: AB_2223021 |
| Rabbit anti-Caldesmon | Abcam | Cat#ab32330; RRID: AB_725810 |
| Rabbit anti-PDGFRα | Abcam | Cat#ab124392; RRID: AB_10978090 |
| Biotin anti-mouse TER-119 | BioLegend | Cat#116204; RRID: AB_313705 |
| Biotin anti-mouse CD45 | BioLegend | Cat#103104; RRID: AB_312969 |
| Biotin anti-mouse CD31 | BioLegend | Cat#102404; RRID: AB_312899 |
| PE/Cy7 anti-mouse/rat CD29 | BioLegend | Cat#102222; RRID: AB_528790 |
| PE anti-mouse CD140a (PDGFR-α) | BioLegend | Cat#135906; RRID: AB_1953269 |
| PerCP/Cy5.5 anti-mouse CD24 | BioLegend | Cat#101824; RRID: AB_1595491 |
| Antibodies (continued) | ||
| Alexa Fluor647 mouse anti-CD9 | BioLegend | Cat#124810; RRID: AB_2076037 |
| Rilliant Violet 421mouse anti-Ly-6C | BioLegend | Cat#128032; RRID: AB_2562178 |
| CD44 rat anti-mouse CD44, V500 | BD Biosciences | Cat#560780; RRID: AB_1937316 |
| Ly-6A/E(Sca-1) rat anti-mouse, V500 | BD Biosciences | Cat#561228; RRID: AB_10584334 |
| PE rat anti-mouse CD34 | BD Biosciences | Cat#551387; RRID: AB_394176 |
| GFP Tag Polyclonal antibody | Thermo Fisher Scientific | Cat#A-21312; RRID: AB_221478 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Matrix Matrigel High Prot | VWR/Genemate | CAS: 80094–330 |
| BODIPY 650/665-X NHS Ester (Succinimidyl Ester) | Invitrogen/Thermo Fisher | CAS: D10001 |
| Recombinant Mouse IGFBP-2 (carrier- free) | Biolegend | CAS: 750304 |
| FITC Streptavidin | Biolegend | CAS: 405202 |
| SYTOX BlUE dead cell stain | Invitrogen | CAS: S34857 |
| DAPI Nuclear Counterstain | Thermo Fisher | CAS: 62248 |
| BMP4 Recombinant Human Protein | Invitrogen | CAS: PHC9534 |
| Rosiglitazone | Sigma | CAS: R2408 |
| Critical Commercial Assays | ||
| IGFBP-2 ELISA Kit, Mouse | Invitrogen | CAS: EMIGFBP2 |
| IGFBP-6 ELISA Kit, Mouse | Invitrogen | CAS: EMIGFBP6 |
| Mouse RBP4 Quantikine ELISA Kit | Fisher Scientific | CAS: MRBP40 |
| Mouse IGF-I ELISA Kit | Sigma | CAS: RAB0229 |
| FITC BRDU FLOW KIT | BD biosciences | 559619 |
| Experimental Models: Organisms/Strains Deposited Data | ||
| C57BL/6J | The Jackson Laboratory | #000664 |
| C57BL/6-Tg(UBC-GFP) | The Jackson Laboratory | #004353 |
| B6.129(Cg)-Gt(ROSA)26SOR (mT/mG) | The Jackson Laboratory | #007676 |
| FVB/NJ | The Jackson Laboratory | #001800 |
| C3H/HeJ | The Jackson Laboratory | #000659 |
| B6.Cg-Lep ob/J (ob/ob) | The Jackson Laboratory | #000632 |
| Oligonucleotides | ||
| Primers – Pparγ: F- GCCTATGAGCACTTCACAAGAAAT | This Paper | N/A |
| Primers – Pparγ: R- GGAATGCGAGTGGTCTTCCA | This Paper | N/A |
| Oligonucleotides (Continued) | ||
| Primers – C/ebpα: F- GCAAAGCCAAGAAGTCGGTG | This Paper | N/A |
| Primers – C/ebpα: R- TCACTGGTCAACTCCAGCAC | This Paper | N/A |
| Primers – Fabp4: F- TGAAATCACCGCAGACGACA | This Paper | N/A |
| Primers – Fabp4: R- ACACATTCCACCACCAGCTT | This Paper | N/A |
| Primers – Col1a1: F- CGCTGGTCAAGATGGTC | This Paper | N/A |
| Primers – Col1a1: R- CTCCAGCCTTTCCAGGTTCT | This Paper | N/A |
| Primers – Col1a2: F- AGCCCTGGTTCTCGAGGT | This Paper | N/A |
| Primers – Col1a2: R- CCGGTTGAACCACGATTG | This Paper | N/A |
| Primers – Col3a1: F- CTCCTGGTGAGCGAGGAC | This Paper | N/A |
| Primers – Col3a1: R- GACCAGGTTGCCCATCACT | This Paper | N/A |
| Primers – Col4a1: F- TGGCACAAAAGGGACGAG | This Paper | N/A |
| Primers – Col4a1: R- GGCCAGGAATACCAGGAAGT | This Paper | N/A |
| Primers – Col4a2: F- CCCGGATCTGTACAAGGGTG | This Paper | N/A |
| Primers – Col4a2: R- CGCCTTTTGAGATTACGCCG | This Paper | N/A |
| Primers – Col5a1: F- CAAGAAGTCTGAGGGAGCCA | This Paper | N/A |
| Primers – Col5a1: R- ACGCTTGAATTCACTGAACCAG | This Paper | N/A |
| Oligonucleotides (Continued) | ||
| Primers – Fbln1: F- GACACCTTCCGCCAAGAGAA | This Paper | N/A |
| Primers – Fbln1: R- ATGATCTCCTCAGGACGGGT | This Paper | N/A |
| Oligonucleotides (continued): See Table S1. | ||
| Deposited Data | ||
| Mass spectrometry data | ProteomeXchange Consortium via the PRIDE [1] partner repository | Accession # PXD014672 |
| Software and Algorithms | ||
| Graphpad Prism 7 | Graphpad | http://www.graphpad.com/scientific-software/prism/ |
| CellSens | Olympus | https://www.olympus-lifescience.com/en/software/cellsens/ |
| GeneTools | Syngene Bioimaging | http://www.syngene.com/genetools/ |
| Graphpad Prism 7 | Graphpad | http://www.graphpad.com/scientific-%20software/prism/ |