Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Toxoplasma gondii) | As listed in Supplementary file 2 | ToxoDB release 53 | Supplementary file 2 | |
| Strain, strain background (T. gondii) |
T. gondii RHΔhxgprtΔku80; RHΔhxgprtΔku80/TIR1 and Supplementary file 1b |
Generous gifts from Prof. Vern Carruthers and Prof. David Sibley PMID:19218426 PMID:28465425 |
See Materials and methods | |
| Strain, 6- to 8-week-old femal, Balb/C (Mus musculus) | Balb/C | Division of Experimental Animals, Beijing University |
Strain code 028 | |
| Recombinant protein | Supplementary file 2c | This paper | Supplementary file 2c | See Materials and methods |
| Softwares, algorithm | PRISM, version 8.0 | http://www.graphpad.com | GraphPAD v8.0 RRID:SCR_002798 |
|
| Softwares, algorithm | InterPro:protein sequence analysis & classification | https://www.ebi.ac.uk/interpro/ | InterPro RRID:SCR_006695 |
|
| Softwares, algorithm | Toxoplasma Genomics Resource |
http://toxodb.org/toxo/ (Alvarez-Jarreta et al., 2024) |
ToxoDB release 53 | |
| Softwares, algorithm | Eukaryotic Pathogen Genomics Resources |
https://veupathdb.org/veupathdb (Alvarez-Jarreta et al., 2024) |
VEuPathDB | |
| Softwares, algorithm | MetaboAnalyst 5.0 |
https://www.metaboanalyst.ca
(Pang et al., 2021) PMID:34019663 |
MetaboAnalyst | |
| Softwares, algorithm | jackhmmer |
http://hmmer.org/
(Prakash et al., 2017) PMID:29220076 |
HMMER v3.3.2 | |
| Softwares, algorithm | trimAL v1.2.rev57 |
https://vicfero.github.io/trimal/
(Capella-Gutiérrez et al., 2009) PMID:19505945 |
trimAL v1.2.rev57 | |
| Softwares, algorithm | TBtools |
https://github.com/CJ-Chen/TBtools
(Chen and Zhao, 2023) PMID:37740491 |
||
| Softwares, algorithm | R v4.2.0 | https://www.r-project.org/ | RRID:SCR_001905 | |
| Softwares, algorithm | ComplexHeatmap |
https://bioconductor.org/packages/release/bioc/html/ComplexHeatmap.html
(Gu et al., 2016) PMID:27207943 |
||
| Softwares, algorithm | ChiPlot | https://www.chiplot.online/ | ||
| Softwares, algorithm | Mafft v7.490 |
https://mafft.cbrc.jp/alignment/software/
(Katoh et al., 2019) PMID:28968734 |
||
| Chemical compound, drug | Pyrimethamine | Sigma-Aldrich | Cat#46706 | 3 µM |
| Chemical compound, drug | Mycophenolic acid | Sigma-Aldrich | Cat#M5255 | 25 µg/ml |
| Chemical compound, drug | Xanthine | Sigma-Aldrich | Cat#X4002 | 25 µg/ml |
| Chemical compound, drug | 3-Indoleacetic acid (auxin) | Sigma-Aldrich | Cat#I2886 | 500 µM |
| Chemical compound, drug | D-biotin | Sigma-Aldrich | Cat#B4639 | 500 µM |
| Chemical compound, drug | 4% paraformaldehyde solution | Solarbio PMID:37108334 PMID:36813769 |
Car#P1110 | |
| Antibody | Mouse anti-Ty (BB2) |
In house hybridoma; gift from Prof. Philippe Bastin |
1:100 | |
| Antibody | Rabbit polyclonal anti-HA (SG77) | Thermo Fisher Scientific | Cat#71-5500 | 1:500 |
| Antibody | Mouse monoclonal anti-HA | BioLegend | Cat#901501 | 1:500 |
| Antibody | Rabbit polyclonal anti-GAP45 | Home-made PMID:36813769 |
Polyclonal | 1:500 |
| Antibody | Rabbit polyclonal anti-actin | Home-made PMID:36813769 |
Polyclonal | 1:1000 |
| Antibody | Rabbit polyclonal anti-IMC1 | Home-made PMID:36813769 |
Polyclonal | 1:1000 |
| Antibody | Rabbit polyclonal anti-ACP | Home-made PMID:36813769 |
Polyclonal | 1:1000 |
| Antibody | Mouse monoclonal anti-GFP | Thermo Fisher Scientific | Cat#MA5-15349 | 1:500 |
| Antibody | Streptavidin Alexa Fluor-488 conjugate | LICOR | Cat#926-3230 | 1:2000 |
| Antibody | Alexa Fluor 488 Goat anti-mouse IgG (H+L) | Thermo Fisher Scientific | Cat#A-11029 | 1:2000 |
| Antibody | Alexa Fluor 568 Goat anti-mouse IgG (H+L) | Thermo Fisher Scientific | Cat#A-11031 | 1:2000 |
| Antibody | Alexa Fluor 568 Goat anti-rabbit IgG (H+L) | Thermo Fisher Scientific | Cat#A-11036 | 1:2000 |
| Antibody | Alexa Fluor 488 Goat anti-rabbit IgG (H+L) | Thermo Fisher Scientific | Cat#A-11034 | 1:2000 |
| Antibody | IRDye 800CW Goat anti-mouse IgG (H+L) | LI-COR | Cat#926-32210 | 1:2000 |
| Antibody | IRDye 800CW Goat anti-rabbit IgG (H+L) | LI-COR | Cat#926-32211 | 1:2000 |
| Antibody | IRDye 680CW Goat anti-rabbit IgG (H+L) | LI-COR | Cat#926-68071 | 1:2000 |
| Antibody | IRDye 680CW Goat anti-mouse IgG (H+L) | LI-COR | Cat#926-68070 | 1:2000 |
| Antibody | IRDye 800CW Streptavidin | LI-COR | Cat#926-32230 | 1:2000 |
| Commercial assay or kit | pEASY-Basic Seamless Cloning and Assembly Kit | Transgene | CU201-02 | |
| Commercial assay or kit | Pierce Streptavidin Magnetic Beads | Thermo Fisher Scientific | Cat#88816 | |
| Commercial assay or kit | ProLong Gold Antifade Mountant without DAPI | Thermo Fisher Scientific | Cat#36930 | |
| Commercial assay or kit | ProLong Gold Antifade Mountant with DAPI | Thermo Fisher Scientific | Cat#36931 | |
| Cell line (include species here) |
Human Foreskin Fibroblasts (human) | ATCC | Cat#SCRC-1041 | |
| Others | Fetal bovine serum (solution) | Transgene PMID:37108334 PMID:36813769 |
FS201-02 | |
| Others | Dulbecco’s modified Eagle medium (DMEM) (powder) | Thermo Fisher Scientific PMID:37108334 PMID:36813769 |
Cat#12800-082 | |
| Others | Penicillin–streptomycin (100×) | Solarbio PMID:37108334 PMID:36813769 |
P1400 | |
| Others | Glutamine (100×) | Solarbio PMID:37108334 PMID:36813769 |
G0200 |