Figure 9. Temporal order of all the proteins examined in the present and previous studies.

(A) Peak-to-peak duration times obtained in our previous (Ishii et al., 2016; Suda et al., 2013; Tojima et al., 2019) and present studies. Time flows from left to right. The lengths of the black horizontal lines with arrows reflect the peak-to-peak times (s). (B) The colored lines show the residence periods of the indicated proteins at a single ERGIC/Golgi/trans-Golgi network (TGN) cisterna. We propose that the endoplasmic reticulum Golgi intermediate compartment (ERGIC)-Golgi maturation process can be divided into following successive stages: the ‘ERGIC/Golgi entry core compartment (GECCO)’ stage, which is defined by the presence of Grh1, Emp46, Ypt1 (first appearance), Rer1, Erd2, and Sed5, the ‘cis-Golgi’ stage, defined by the presence of Mnn9, Gea1, Vrg4, and Sec21, the ‘medial-Golgi‘ stage, defined by the presence of Mnn2, Gnt1, and Sec21, and the ‘trans-Golgi‘ stage, defined by the presence of Sys1, Imh1, Ypt6, Ypt1 (second appearance), Gea2, and Apl6. In addition, we previously defined the ‘early TGN stage’ and the ‘late TGN stage’ (Tojima et al., 2019).