Table 1. Peak-to-peak duration time between the maximum fluorescence intensities of the two proteins at single cisterna.
| No. | Labeled proteins | Peak-to-peak duration (s) (mean ± SD) | *Number of puncta (n) in total cells (N) | Figure No. | |
|---|---|---|---|---|---|
| n | N | ||||
| 1 | EGFP-Emp46 → Mnn9-mCherry | 31.0±24.1 | 10 | 6 | Figure 1A–E; Figure 1—figure supplement 1A and F |
| 2 | GFP-Ypt1 → Mnn9-mCherry | 29.5±14.6 | 10 | 8 | Figure 1F–J; Figure 1—figure supplement 1B and F |
| 3 | EGFP-Rer1 → Mnn9-mCherry | 13.2±11.7 | 14 | 9 | Figure 1K–N; Figure 1—figure supplement 1C and F |
| 4 | Erd2-GFP → Mnn9-mCherry | 16.4±10.3 | 18 | 14 | Figure 1O–R; Figure 1—figure supplement 1D and F |
| 5 | EGFP-Emp46 → iRFP-Rer1 | 3.9±9.0 | 31 | 15 | Figure 1—figure supplement 1S–V; Figure 1—figure supplement 1E and F |
| 6 | Grh1-2xEGFP → Mnn9-mCherry | 37.1±16.8 | 14 | 12 | Figure 2A–D; Figure 2—figure supplement 1A and D |
| 7 | Grh1-2xmCherry → EGFP-Rer1 | 26.2±17.7 | 13 | 9 | Figure 2E–H; Figure 2—figure supplement 1B and D |
| 8 | Grh1-2xmCherry → GFP-Ypt1 | 18.6±19.4 | 18 | 15 | Figure 2I–L; Figure 2—figure supplement 1C and D |
| 9 | EGFP-Sed5 → Mnn9-mCherry | 1.2±11.8 | 13 | 10 | Figure 3A–D; Figure 3—figure supplement 1A and E |
| 10 | Mnn9-mCherry → GFP-Vrg4 | 11.9±11.6 | 18 | 13 | Figure 3E–H; Figure 3—figure supplement 1B and E |
| 11 | Mnn9-mCherry → Sec21-2xEGFP | 37.9±33.2 | 17 | 13 | Figure 3I–L; Figure 3—figure supplement 1C and E |
| 12 | Mnn9-mCherry → Mnn2-GFP | 18.3±13.3 | 6 | 3 | Figure 3M–P; Figure 3—figure supplement 1D and E |
| 13 | Sys1-iRFP → GFP-Ypt1 | 19.7±16.3 | 18 | 13 | Figure 6A–D; Figure 6—figure supplement 1A and C |
| 14 | GFP-Ypt1 → Sec7-iRFP | 26.4±28.5 | 7 | 5 | Figure 6E–H; Figure 6—figure supplement 1B and C |
| 15 | Gea1-EGFP → Mnn9-mCherry | 6.0±10.0 | 15 | 9 | Figure 7A–D; Figure 7—figure supplement 1A and F |
| 16 | Mnn9-mCherry → Gea2-GFP | 76.2±21.7 | 13 | 11 | Figure 7E–H; Figure 7—figure supplement 1B and F |
| 17 | Gnt1-GFP → Gea2-2xmCherry | 30.0±12.4 | 15 | 12 | Figure 7I–L; Figure 7—figure supplement 1C and F |
| 18 | Gea2-2xmCherry → Tlg2-GFP | 9.3±11.0 | 21 | 11 | Figure 7M–P; Figure 7—figure supplement 1D and F |
| 19 | Gea2-GFP → Sec7-tagRFP | 23.6±16.8 | 7 | 6 | Figure 7Q–T; Figure 7—figure supplement 1E and F |
| 20 | Sys1-iRFP → Imh1-EGFP | 0.6±12.9 | 16 | 10 | Figure 8A–D; Figure 8—figure supplement 1A and D |
| 21 | Sec7-tagRFP → Gga1-GFP | 15.8±17.9 | 13 | 6 | Figure 8E–H; Figure 8—figure supplement 1B and D |
| 22 | Sys1-iRFP → Apl6-GFP | 5.8±27.8 | 13 | 11 | Figure 8K–O; Figure 8—figure supplement 1C and D |
| †23 | Sec7-mRFP → Ypt32-GFP | 51.9±23.2 | 7 | 4 | – |
*
Number of puncta and cells used for the calculation of peak-to-peak duration. In each cell, 1–4 puncta were selected for the calculation.
†
Suda, Y., Kurokawa, K., Hirata, R., and Nakano, A. 2013. Rab GAP cascade regulates dynamics of Ypt6 in the Golgi traffic. Proc. Natl. Acad. Sci. U.S A. 110:18976–18981.