Table 2.

Blocking peptides and amine used for preabsorption in the negative control reactions.

Blocking peptides and amine	Source and code	Concentration	Preabsorption
CGRP	Bachem AG [Bubendorf, CH], H4924	10 μg mL−1	24 h at 4°C
NPY	Bachem AG [Bubendorf, CH], H6375	10 μg mL−1	24 h at 4°C
nNOS	Santa Cruz Biotechnology, Inc. [Santa Cruz, CA, USA], sc648P	100 μg mL−1	16–18 h at R. T.
5-HT	Sigma-Aldrich [St. Louis, MO, USA], H9523	100 μg mL−1	16–18 h at R. T.
SP	Bachem AG [Bubendorf, CH], H1890	10 μg mL−1	24 h at 4°C
VIP	Sigma-Aldrich [St. Louis, MO, USA], V3628	100 μg mL−1	16-18 h at R. T.

CGRP, calcitonin gene-related peptide; NPY, neuropeptide Y; nNOS, neuronal nitric oxide synthase; 5-HT, 5-hydroxytriptamine (serotonin); SP, substance P; VIP, vasoactive intestinal peptide; R. T., room temperature.