Figure 5.

Basal Myogenic Expression Is Dysregulated in Rats and Patients With HFpEF

Skeletal muscle gene expression of PAX7, Myf5, MyoD, myogenin, myomaker, apelin, APLNR, Gli2, PTCH, piezo1, and IGF1 measures in (A) rat EDL (CON n = 4, HFpEF n = 4) and (B) vastus lateralis biopsies from healthy age-matched control subjects (CON n = 10) and patients with diagnosed HFpEF (n = 10) measuring protein expression of Pax7 and MyoD normalized to loading control (GAPDH) with representative blots presented. Differences between groups were analyzed by unpaired 2-tailed Student t-tests. Data are presented as mean ± SD, and the level of significance was accepted as ∗P < 0.05, ∗∗ P< 0.01, ∗∗∗P < 0.001 for all analyses. Abbreviations as in Figures 1 and 2.