Skip to main content
. 2023 Nov 6;6(1):55–66. doi: 10.1016/j.jaccao.2023.10.002

Figure 2.

Figure 2

Changes in the NGF-TRKA Axis After MI in Mice

(A, B) Serum nerve growth factor (NGF) concentration at (A) 2 weeks and (B) 5 weeks after sham or myocardial infarction (MI) surgery (A: sham = 6, MI = 11; B: sham = 11, MI = 11). (C, D) Messenger RNA (mRNA) expressions of Ngf in the heart at the border area (MI -B) (n = 9 in each, C) and the remote area (MI -R) (n = 6 in each, D) 3 days after MI. (E, F) NGF protein expressions in the heart at (E) the border area (MI-B) and (F) the remote area (MI-R) 3 days after MI. The densitometric analyses are shown in the graphs (n = 6 in each). (G) Tropomyosin receptor kinase A (TRKA) and AKT phosphorylation in tumor tissues of the sham-operated and MI-operated mice. The ratios of phosphorylated TRKA (p-TRKA) to total TRKA and phosphorylated AKT (p-AKT) to total AKT are quantified, and the densitometric analyses are shown in the graphs (n = 5 in each). GAPDH was used as the loading control. Data are presented as mean ± SEM. ∗P < 0.05 and ∗∗∗P < 0.001, determined by 2-tailed unpaired Student’s t test.