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CLEC2D was effectively knocked down after transfection of its shRNAs in ccRCC cells. mRNA expression (qPCR) (A) and protein expression (WB) (B) of CLEC2D after transfection in 786-O and Caki-2 cells with shRNA and empty vector, respectively, were detected. Colony formation assays (C) and CCK-8 proliferation assays (D) were performed to detect the effects of CLEC2D on ccRCC cell proliferation.
