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. 1998 Mar;72(3):2113–2124. doi: 10.1128/jvi.72.3.2113-2124.1998

FIG. 8.

FIG. 8

Mutation of the TATA box strongly represses HPV-18 URR activity in HeLa cells. CAT assays were carried out with extracts prepared from HeLa cells transfected with plasmids p18URR (wild type), p18URR-22M1 (containing the mutated switch sequence OL22M1), p18URR-TATAM1 (containing the mutated TATA sequence TATA-M1), and p18URR-22M1-TATAM1 (containing the mutated switch sequence and a mutated TATA sequence). HeLa cells were transfected with 5 μg (lanes 1 to 4) or 3 μg (lanes 5 to 8) of DNA from each plasmid, as indicated in the figure, together with 0.6 μg of RSV/L (18) as an internal control. CAT activities were quantified relative to the activity obtained with p18URR (5 μg of transfected DNA) (lane 1), which was set at 1 (for 3 μg of transfected DNAs [lanes 5 to 8], the activities quantified relative to p18URR [set at 1] are shown in parentheses). The results of a representative CAT assay are shown. Relative CAT activities were as follows: p18URR (lane 1), 1.00; p18URR-TATAM1 (lane 2), 0.10; p18URR-22M1 (lane 3), 0.51; p18URR-22M1-TATAM1 (lane 4), 0.04; p18URR (lane 5), 0.447 (1); p18URR-TATAM1 (lane 6), 0.025 (0.056); p18URR-22M1 (lane 7), 0.15 (0.34); and p18URR-22M1-TATAM1 (lane 8), 0.014 (0.031).