FIG. 2.

Analysis of intermolecular integration and autointegration products by restriction enzyme digestion. (A) Map of the MoMuLV genome and sizes of DNA fragments from substrates (viral cDNA and φX174 RFI), integration product (II), and autointegration products (circular) expected to be detected by the LTR probe. (B to D) Southern blot analyses of integration products generated by PICs partially purified by low-ionic-strength precipitation (lanes 1 and 2) or salt-stripped PICs (lanes 3 and 4) in the absence (−) or presence (+) of linearized target DNA (B) or circular target DNA (C and D). DNAs were digested with HindIII (C) or BamHI (D) prior to electrophoresis.