Fig. 4.

The levels of S-adenosylmethionine (SAM) were elevated in the D. desulfurican-treated chemotherapy model group. (a, b, and c) Comparison of serum metabolomic profiles between the ABX-Folfox and ABX-Folfox-Dsv groups in mice: (a) volcano plot, (b) mass-to-charge ratio vs. p-value scatter plot, and (c) heatmap of differential metabolites. Metabolomics analysis was conducted using non-targeted LC-MS/MS. years (n = 6 mice/group). ELISA was performed to measure the SAM concentration in the AF and AFD groups. (d) Concentration of SAM in serum; (e) Concentration of SAM in mouse faeces; (f) Concentration of SAM in mouse caecal contents; (g) Concentration of SAM in mouse tumour tissues. years (n = 6 mice/group). The results are presented as mean ± SEM. ∗p < 0.05. Statistical significance was determined using a one-way ANOVA.