SAM Impairs the efficacy of FOLFOX chemotherapy. (a) The mRNA expression levels of c-Cyc and CycD after SAM combined with chemotherapy treatment in CRC cells. (b) Compared to the FOLFOX group, the combination of SAM with FOLFOX enhanced the viability of MC38, HCT116, and DLD1 CRC cells. (c) Compared with the FOLFOX group, the combined use of SAM and FOLFOX enhanced colony formation in CRC cells. (d) Compared to the FOLFOX group, the combination of SAM and FOLFOX enhanced the proliferative ability of CRC cells. (e, f) In the Folfox + SAM group, both the migration (e) and invasion (f) abilities of the CRC cells were higher than those in the Folfox group. (g)The apoptosis rates of the three types of CRC cells in the Folfox + SAM group were lower than those in the Folfox group. Data were from one representative of three independent experiments. The results are presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, ∗∗∗∗p < 0.001. Statistical significance was determined using one-way or two-way analysis of variance (ANOVA).