Fig. 3. uPAR CAR T cells eliminate senescent cells in old mice.
a, Experimental scheme for Figs. 3–5. C57BL/6N mice (18 to 20 months old) were injected with 0.5 × 106 m.uPAR-m.28z CAR T cells, h.19-m.28z CAR T cells or UT cells generated from CD45.1 mice 16 h after administration of cyclophosphamide (200 mg per kg body weight). Mice were harvested 20 d later and/or monitored over time. Schematic created with BioRender.com. b, Representative SA-β-gal and uPAR staining 20 d after cell infusion. c, Heat map depicting fold change in the levels of SASP cytokines compared to UT-treated mice (n = 3 for UT cells; n = 3 for h.19-m.28z; n = 4 for m.uPAR-m.28z). Results are from one experiment (b and c).