Fig. 5. uPAR CAR T cells revert natural age-associated phenotypes.
Mice were treated with m.uPAR-m.28z CAR T cells, h.19-m.28z CAR T cells or UT cells as schematized in Fig. 3a. a, Levels of basal glucose (mg ml−1) after starvation 2.5 months after cell infusion (n = 11 mice for UT; n = 12 for h.19-m.28z and for m.uPAR-m.28z). b, Levels of glucose before (0 min) and after intraperitoneal administration of glucose (2 g per kg body weight) 2.5 months after cell infusion (samples sizes as in a). c, Area under the curve (AUC) representing the results from b. Each point represents a single mouse. d, Levels of insulin before and 15 min after intraperitoneal glucose administration (2 g per kg body weight) 2.5 months after cell infusion (n = 6 for UT; n = 5 for h.19-m.28z; n = 6 for m.uPAR-m.28z). e, Fold change in time to exhaustion in exercise capacity testing before cell infusion and 2.5 months after it (n = 7 for UT; n = 8 for h.19-m.28z and n = 8 for m.uPAR-m.28z). f, Fold change in maximum speed in capacity testing before cell infusion and 2.5 months after it (sample sizes as in e). g,h, Percentage of CD45.1+ T cells in the spleen (g) or liver (h) of 4-month-old or 20-month-old mice 20 d after cell infusion (n = 3 mice per age group for UT and for h.19-m.28z; n = 4 for m.uPAR-m.28z). The corresponding flow cytometry gating is shown in Extended Data Fig. 10. Results are from two independent experiments (a–c, e and f) or one experiment (d, g and h). Data are the mean ± s.e.m.; P values from two-tailed unpaired Student’s t-test (a, c, d, g and h) or two-tailed Mann–Whitney test (e and f).