TABLE 2.
Spontaneous 3′-NCR deletions detected in a panel of recombinant TBE virus mutants
| Virusa | No. of passagesb | No. of deletions in suckling micec | Adult miced
|
|
|---|---|---|---|---|
| No. testede | No. of deletionsf | |||
| R-1 | 1 | 0 | 2 | 0 |
| R-2 | 1 | 0 | 0 | 0 |
| R-3 | 1 | 0 | 2 | 0 |
| R-4 | 1 | 0 | 1 | 0 |
| R-5 | 1 | 0 | 0 | 0 |
| R-6 | 1 | 0 | 2 | 0 |
| R-7 | 1 | 0 | 8 | 2 (10394–10604, 10441–10537) |
| R-8 | 1 | 0 | 0 | 0 |
| R-9 | 1 | 0 | 0 | 0 |
| R-10 | 1 | 0 | 1 | 0 |
| R-11 | 5 | 0 | 1 | 1 (10450–10620) |
| R-12 | 5 | 0 | 1 | 1 (10494–10596) |
| R-13 | 5 | 0 | 2 | 1 (10495–10574) |
| R-14 | 5 | 2 (10458–10689, 10458–10811) | 2 | 2 (10504–10794, 10453–10603) |
| R-15 | 5 | 0 | 1 | 0 |
| R-16 | 5 | 1 (10485–10552) | 1 | 1 (10454–10592) |
| R-17 | 9 | 0 | 0 | 0 |
| Total | 3 | 24 | 8 | |
a
Recombinant TBE virus Neudoerfl mutants bearing specific mutations in regions of the genome other than the 3′ NCR, consecutively numbered.
b
Number of BHK cell culture passages (at low MOI) prior to the generation of suckling-mouse brain stock suspensions.
c
Number of deletion mutants found in suckling-mouse brain stock suspensions; boundaries of deletions (positions of the first and last deleted nucleotides) are in parentheses.
d
Virus isolated from the brains of adult mice after infection with the suckling-mouse brain stock suspensions.
e
Number of viral isolates tested.
f
Number of deletion mutants found; boundaries of deletions in parentheses.