TABLE 3.
Stock virus preparations and plaque morphologies for TBE virus mutants with engineered 3′-NCR deletions
| Plasmid with 3′-NCR deletion | Mutant virus | Infectivity titer of stock solution (PFU/ml) | Plaque morphologya |
|---|---|---|---|
| pTNd/3′Δ10795 | R-Nd/3′Δ10795 | 3 × 108 | WT |
| pTNd/3′Δ10826 | R-Nd/3′Δ10826 | 1 × 108 | WT |
| pTNd/3′Δ10847 | R-Nd/3′Δ10847 | 3 × 108 | WT |
| pTNd/3′Δ10870 | R-Nd/3′Δ10870 | 1 × 108 | WT |
| pTNd/3′Δ10919 | R-Nd/3′Δ10919 | 3 × 106 | Turbid |
| pTNd/3′Δ10994 | Not viable | ||
| Neudoerflb | 3 × 108 | WT | |
| R-Neudoerflb | 3 × 108 | WT |
a
Determined at 37 and 40°C; morphologies were identical at both temperatures. WT, wild-type (strain Neudoerfl) morphology.
b
Control viruses.