Table 2.

Comparison of the average difference in ∆G⁰ values (∆G⁰_all −∆G⁰_wide) obtained from all the PMF plots with different considerations.

Analysis Method	Mean Difference (kJ/mol)	p-value t-test	p-value Wilcoxon
Results using the mean values for each system
Without Uncertainties	−2.67 ± 1.10	0.028	0.0125
With Uncertainties	−1.96 ± 1.04	0.080