Table 2.
Enriched GO terms and KEGG pathways of the differentially expressed genes (DEGs) in the intersection groups. Group (SP-A) Genes showing increased expression across stages in N. Steck and decreased expression across stages in the other genotypes. (SP-B) Genes with increased expression in N. Steck in relation to the other genotypes in maturation stage. (SP-AB) Intersection of genes from SP-A and SP-B. Group (SN-A) Genes exhibiting increased expression across stages in N. Rosada and N. Branca and reduced expression, across stages, in N. Steck. (SN-B) Genes with increase expression in N. Rosada and N. Branca in relation to N. Steck in maturation stage. (SN-AB) Intersection of genes from SN-A and SN-B.
| Group | Comparison | DEG | Quantity | Gene ontology (GO) enrichment | KEGG pathway enrichment |
|---|---|---|---|---|---|
| SP-A |
NBM vs NBV NRM vs NRV NSM vs NSV |
Down Down Up |
282 | Response to auxin (GO:0009733), circadian rhythm (GO:0007623), endoplasmic reticulum to cytosol auxin (GO:0080162), and auxin-activated signaling pathway (GO:0009734) | Plant hormone signal transduction (vvi04075) |
| SP-B |
NSM vs NRM NSM vs NBM |
Up Up |
607 | Response to biotic stimulus (GO:0009607), phenylpropanoid biosynthetic process (GO:0009699), and dolichol-linked oligosaccharide biosynthetic process (GO:0006488) | Phenylpropanoid biosynthesis (vvi00940), stilbenoid, diarylheptanoid and gingerol biosynthesis (vvi00945), and phenylalanine metabolism (vvi00360) |
| NBM vs NBV | Down | ||||
| NRM vs NRV | Down | ||||
| SP-AB | NSM vs NSV | Up | 100 | Transmembrane transport (GO:0055085) | Plant hormone signal transduction (vvi04075) |
| NSM vs NBM | Up | ||||
| NSM vs NRM | Up | ||||
| SN-A |
NBM vs NBV NRM vs NRV NSM vs NSV |
Up Up Down |
1,343 | Translation (GO:0006412), plant-type secondary cell wall biogenesis (GO:0009834), regulation of DNA-templated transcription (GO:0006355), protoporphyrinogen IX biosynthetic process (GO:0006782), and chlorophyll biosynthetic process (GO:0015995) | Phenylpropanoid biosynthesis (vvi00940), flavonoid biosynthesis (vvi00941) and photosynthesis (vvi00195) |
| SN-B |
NSM vs NBM NSM vs NRM |
Down Down |
97 | Sterol biosynthetic process (GO:0016126) and recognition of pollen (GO:0048544) | ABC transporters (vvi02010), cutin (vvi00073), suberin and wax biosynthesis and flavonoid biosynthesis (vvi00941) |
| NBM vs NBV | Up | ||||
| NRM vs NRV | Up | ||||
| SN-AB | NSM vs NSV | Down | 22 | Sterol biosynthetic process (GO:0016126) and wax biosynthetic process (GO:0010025) | Cutin, suberin and wax biosynthesis (vvi00073) |
| NSM vs NBM | Down | ||||
| NSM vs NRM | Down |