Figure 10.
Synergistic effects of #33 and suramin on Nedd8-CRL1/SCFβ-TrCP-dependent β-catenin ubiquitination. #33 or suramin, alone or in combination as indicated, were added to the reaction. The detailed procedure is described under “Experimental Procedure.” The substrate levels after reactions were quantified and shown. In lane 2 without compound, the remaining substrate level is 18% of the input. “18%” was subtracted from the remaining substrate level in reactions with various combinations of the compounds, resulting in a set of “background-subtracted” numbers as shown. This experiment was repeated once.