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Effects of compound KH-4-43 on CRL1/SCFβ-TrCP-dependent β-catenin ubiquitination.A, reactions with Nedd8-CRL1/SCFβ-TrCP. Incubation is 10 min at 37 °C. B, reactions with CRL1/SCFβ-TrCP. Incubation is 20 min at 37 °C. The detailed procedure is described under “Experimental Procedure.” The substrate levels after reactions were quantified and shown. These experiments were repeated once.
