FIG. 1.
Features of plasmids which allow the rescue of the chimeric viruses MGV and MG/FV. The entire MV F and H open reading frame and parts of the untranslated region were substituted by the open reading frame of VSV G. The nucleotides in lowercase are derived from the VSV G gene, and those in uppercase represent MV sequences. The numbering of the nucleotides refers to the MV antigenomic sequence as given by Radecke and Billeter (27). The underlined nucleotides were added during the cloning procedure to comply with the rule of six (4) for p(+)MG/FV and p(+)MGV. The antigenomic sense of the specified RNAs is indicated by (+). Relevant regions of the nucleotide sequences near the ends of the VSV inserts and of the corresponding encoded amino acids are detailed. The sequence tags (MV 1702 and 1805/6) are present in all plasmids. Boxes around nucleotides indicate the nontranscribed gene boundary trinucleotides between genes M and F and between genes H and L, respectively. The MV cDNA sequence is available from the EMBL gene bank under accession no. Z66517.