FIG. 5.

Purification and characterization of MGV and MG/FV particles in comparison to MV and VSV. (A) Virus particles were first pelleted on 20% (1.0 ml) and 60% (1.0 ml) sucrose cushions. The interphases were taken and diluted to 20% sucrose, applied to 20 to 60% sucrose gradients, and centrifuged to equilibrium. Fractions were analyzed by SDS-PAGE (12.5% polyacrylamide), immunoblotted, and probed with the antibodies indicated on the right. (B) The blots were reprobed with anti-MV M antibody in addition to anti-VSV. The sucrose density was determined by weighing 100 μl of each fraction. (C) Representative profile of the gradient.