Fig. 8.

3D representations of CN projections to the brainstem and thalamus in the mouse visualized using recombinant anterogradely transported adeno-associated virus (rAAV) as a tracer injected in different parts of the CN. Panels from top to bottom depict a lateral, dorsal, and caudal transparent 3D view with a final section showing the injection site. a Injection centered on the Med, depicting prominent bilateral terminal labeling in the vestibular nuclei and medial reticular formation. Note the conspicuous intracerebellar course of the uncinate fascicle. b Injection centered on the IntA. Apart from the course of labeled fibers to the midbrain and thalamus by way of the scp, note the prominent course of ipsilaterally descending fibers, which seems to be a special feature of rodent connectivity. c Injection centered on the Lat. Here, aspects of an ipsilateral descending tract together with a contralateral descending tract terminating in the medullary reticular formation can be appreciated. See text for further explanation. Yellow, cyan, and magenta circles indicate the approximate sites of the Med, IntA and Lat injections respectively, in the 3D representations. Double arrowheads in the third row point to the nucleocortical projections seen in all cases. Abbreviations: cdt, contralateral descending tract; dfbt, direct fastigiobulbar tract; IC, inferior colliculus; idt, ipsilateral descending tract; IntA, anterior interposed nucleus; Lat, lateral cerebellar nucleus; Med, medial cerebellar nucleus; SC, superior colliculus; scp, superior cerebellar peduncle; Th, thalamus; un, uncinate fascicle; based on material from [145], experiment numbers 268389532, 120, 493, 315, 127, 650, 431)