表3. mNGS与常规方法、RQ-PCR对耶氏肺孢子菌肺炎(PJP)患者的诊断效能比较.
方法 | 敏感性(95% CI) | 特异性(95% CI) | PPV(95% CI) | NPV(95% CI) |
mNGS | 100.0%(69.9%~100.0%) | 98.8%(92.8%~99.9%) | 92.3%(62.1%~99.6%) | 100.0%(94.6%~100.0%) |
常规方法 | 8.3%(0.4%~40.2%)a | 100.0%(94.7%~100.0%)b | 100.0%(5.5%~100.0%)b | 88.7%(80.2%~94.5%)a |
RQ-PCR | 91.6%(59.8%~99.6%)b | 100.0%(94.7%~100.0%)b | 100.0%(67.9%~100.0%)b | 98.9%(92.9%~99.9%)b |
注 mNGS:宏基因组二代测序;RQ-PCR:实时定量-聚合酶链反应;PPV:阳性预测值;NPV:阴性预测值。与mNGS比较,aP<0.05;与mNGS比较,bP>0.05