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. 2024 Mar 19;14:6647. doi: 10.1038/s41598-024-55917-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SV2B Immunofluorescence analysis. (a) There was no specific binding observed in the negative condition of the experiment. (b) Uniform staining across U87MG cells with beta-actin (positive control). (c) Uniform staining across U251MG cells with beta-actin (positive control). (d) The U87MG cell line exhibited intense SV2B staining (~ 1300–2200 range) enriched around the nucleus. (e) U251MG cells exhibited lower intensity of staining for SV2B (~ 900–2000). All microscopic images were taken under × 40 magnification.