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. 2024 Jan 18;130(6):908–924. doi: 10.1038/s41416-023-02522-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Gene Set Enrichment Analysis (GSEA) revealed overrepresented pathways enriched in bulk lung mets (a) and in M-cluster (cluster 2) (b) indicating a clear predilection for OXPHOS over glycolysis in cluster 2. c, d PyMT1/GPx2 KD primary tumour and lung mets primary tumour cells were assayed for OCR and ECAR; normalised OCR data comparing both groups were derived for each of the mitochondrial respiration steps after 1 μM oligomycin, 1 μM FCCP, and 0.5 μM rotenone/antimycin treatment; normalised ECAR values were derived after sequential treatment with 20 mM glucose, 1 μM oligomycin, and 50 mM 2-DG; ****p < 0.0001. e, f Co-staining for GLUT1 (blue), pAMPK (green), VIM (red), and E-CAD (purple) shows individual and overlay staining on GPx2 KD primary tumour (n = 8) and paired lung mets (n = 4) from 4 mice. Representative images are shown.