Fig. 5. CMV infection of cerebral organoids results in re-localisation of DYRK and SHH proteins but no strong change in gene expression.

Cerebral organoids at 55 days of age were infected for 14 days with CMV (Merlin strain). a Immunohistochemistry was performed on FFPE sections. CMV infection induced re-localisation of DYRK and SHH proteins in cerebral organoids, particularly in regions of syncytia. CMV induced re-localisation of DYRK1A, ULK3, and Shh to the cytoplasm and DYRK1B, GLI2, and Rb to the nucleus. Scale bars represent 25 µm. b Organoids were infected with CMV or left untreated (mock) in quadruplicate for bulk RNA-sequencing. Heat map of the 9 DYRK-associated genes that were identified in CMV-infected organoids through RNA-sequencing. c Heat map of the 8 significantly differentially expressed SHH genes. See also Supplementary Data 1, Supplementary Data 4.