Table 2.

Statistical model, advantages, disadvantages and applications of Deseq2 and edgeR pipelines.

Features	DESeq2 [51]	edgeR [52]
Statistical Model	Negative binomial model	Negative binomial model
Advantages	Suitable for data sets with minor variability; user-friendly interface	Speed and robust approach for small data sets
Disadvantages	Lower speed; Less sensitivity for data sets with high biological variability	Reduced sensitivity for data sets with low biological variability
Typical Applications	Analyses under controlled experimental conditions with well-defined control groups [53].	Analysis of complex datasets with high variability [54].