Figure 3.

GroEL particle distribution in the vitrified sample with different concentrations of FFC8. A– D Each alphabetized panel contains six numericized sub-sections ( e. g., Panels A1 through A6 or D1 through D6), organized in the same manner for all panels. Panels A1 through A6 pertain to no-surfactant conditions, and Panels B through D pertain to different FFC8 concentrations as indicated. The first three sub-sections (1–3) show the top, center, and bottom slice of the tomogram from cryoET, respectively, viewed from the top of the grid. Sub-section 4 shows the schematic of Sub-sections 1–3, viewed from the side of the grid. The dashed border (black, orange, and green) in Sub-sections 1–3 corresponds to the dashed horizontal line (black, orange, and green) in Sub-section 4. Sub-section 5 shows the actual particle distribution of the tomogram, viewed from the side, with green dots representing GroEL particles and red horizontal lines representing the AWIs. Sub-section 6 shows the histogram of particle distribution across the entire ice thickness. The X-axis is divided into ranges based on the magnitude of deviation (represented in %) from the center of the ice ( e. g., 0% indicating no deviation from the center of the ice and 100% representing the maximum deviation from the center, i. e., either side of AWI). The Y-axis represents the number of particles (represented as the percentage of the total population) residing in each ice region