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. 2023 Oct 31;9(5):232–240. doi: 10.52601/bpr.2023.230013

Table 2. Low bFGF ESC culture medium (LCDF12).

Composition Volume Final concentration
DMEM/F12 385 mL
KnockOutTM SR-Multi-Species 100 mL 20% (v/v)
GlutaMAXTM-1 5 mL 2 mmol/L
MEM NEAA 5 mL 0.1 mmol/L
Penicillin Streptomycin 5 mL 1% (v/v)
2-Mercaptoethanol 0.5 mL 0.1% (v/v)
bFGF (50 μg/mL) 50 μL 5 ng/mL
PlasmocinTM Treatment 50 μL 0.01% (v/v)