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| Composition | Volume | Final concentration |
| High glucose DMEM | 475 mL | |
| Horse serum | 10 mL | 2% (v/v) |
| GlutaMAXTM-1 | 5 mL | 2 mmol/L |
| MEM NEAA | 5 mL | 0.1 mmol/L |
| Penicillin Streptomycin | 5 mL | 1% (v/v) |
| PlasmocinTM Treatment | 50 μL | 0.01% (v/v) |
