Table 8. Troubleshooting table.

Description	Possible reason	Suggestion
No sufficient hMPCs to initiate subsequent differentiation	Pluripotent cells might contain a substantial population of differentiated cells.	1. Pluripotent cells must be homogeneous without a substantial population of differentiated cells. 2. The starting population of hESCs or iPSCs for differentiation toward hMPCs should be increased.
Low differentiation efficiency of myotube	The hMPC differentiation potential is limited.	At the beginning of myotube differentiation, early-passage hMPCs with higher differentiation potential should be used.
Failure to observe senescence-related phenotypes	Myotube cells are treated and/or collected at inappropriate time points.	It’s necessary to evaluate the senescence phenotypes at multiple time points after initiation of myotube differentiation.