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. 2024 Mar 5;29(2):227–234. doi: 10.1016/j.cstres.2024.03.002

Fig. 2.

Fig. 2

The phosphorylation level of ERKs in THP-1 cells after incubation with DC-SIGN-targeting molecules. (a) Representative results of Western blotting indicating the phosphorylation level of ERKs. After incubation with PMA and IL-4, THP-1 cells were subsequently incubated with recombinant ICAM3, SARS-CoV-2 S1, SAP, mannan, or mannosylated BSA for 1 and 2 h. Then, the cells were lysed, and the 20 µg of total proteins were analyzed. The band intensities of phospho-ERKs relative to total-ERKs were measured, and they were normalized to the control results. GAPDH is a loading control. (b) The phosphorylation levels of ERKs in three independent experiments are summarized. The band images of two additional times of Western blotting are shown in Supplementary Figure S1. *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations used: BSA, bovine serum albumin; DC-SIGN, dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin; ERK, extracellular signal-regulated kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM3, intracellular adhesion molecule 3; IL-4, interleukin-4; ns, not significant; PMA, phorbol 12-myristate 13-acetate; SAP, serum amyloid P.