Fig. 3.

The phosphorylation level of NF-κB p65 in THP-1 cells after incubation with DC-SIGN-targeting molecules. (a) Representative results of Western blotting indicating the phosphorylation level of NF-κB p65. The band intensities of phospho-p65 relative to total-p65 were measured, and were normalized to the control results. GAPDH is a loading control. (b) The phosphorylation levels of NF-κB p65 in three independent experiments are summarized. The band images of two additional times of Western blotting are shown in Supplementary Figure S2. Abbreviations used: BSA, bovine serum albumin; DC-SIGN, dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM3, intracellular adhesion molecule 3; ns, not significant; SAP, serum amyloid P.