Fig. 4.

Comparison of the number of 5-HT-positive neurons and fibres in the whole brain.

The numbers of positive neurons per unit area (27,500 μm²) in different brain areas are shown. n = 3 biologically independent images per group. The data are shown as the means ± SEMs. Student's t-test was used to analyse the differences between the two groups; *P < 0.05, ns: P > 0.05. Source data and P values for different brain areas are shown in Supplementary Table 1. The definitions of the abbreviations for the different brain regions are provided in Table 4.